Research groups
Research
My research is mostly concerned with the application of high-resolution multinuclear (1H, 13C, 15N) og multidimensional (2D/3D) NMR-spectroscopy of isotopically enriched (13C, 15N) proteins in solution and 2D NMR spectroscopy of proteinbinding peptides. The NMR spectra can give us information about structure of molecules, dynamical processes (exchange) and interactions between for instance larger biomolecules and smaller ligand molecules. I am mainly interested in the methodological aspects of NMR spectroscopy, and I am working with NMR spectroscopy applied to many areas within chemistry.
Teaching
Teaching
I am currently teaching the following courses:
Master projects
NMR spectroscopy of proteins and peptides: Master projects are offered within the application of high resolution multinuclear (1H, 13C, 15N and others) and multidimensional (1D, 2D and 3D) NMR spectroscopy applied to proteins, peptides and smaller molecules that are interacting with proteins. NMR spectroscopy can give us important information about the function and working mechanisms of the protein at the molecular level in solution. This include structural information about the active sites, the binding of peptides and smaller molelcules (such as substrates, cofactors, inhibitors and drug molecules) and dynamical processes (the exchange of smaller ligand molecules). The choise of NMR-methods and post-processing procedures will depend strongly on the kind of information sought in each case.
This research is a cooperation with the department of Molecular Biology.
Publications
Masters thesis
- Vikestad Vikøren, Kajsa; Jordheim, Monica; Seddon, Alistair William Robin et al. (2024). Qualitative and quantitative analyses of unbound phenolic compounds from pollen grains of pine (Pinus uncinata). (external link)
- Lien, Lars Elstad; Frøystein, Nils Åge; Andersen, Øyvind M (2015). PELARGONIDIN-3-GLUCOSIDE: AN APPROACH TO STRUCTURE ELUCIDATION BY NMR - Choosing the right experiments. (external link)
Academic article
- Dobrovolska, Olena; Strømland, Øyvind; Handegård, Ørjan Sele et al. (2021). Investigating the Disordered and Membrane-Active Peptide A-Cage-C Using Conformational Ensembles. (external link)
- Drageset, Audun; Frøystein, Nils Åge; Törnroos, Karl Wilhelm et al. (2019). A two-step telescoped continuous flow switchable process leading to nitriles, diaziridine or hydrazine derivatives. (external link)
- Quadri, Coralie Celine; Ralte, Lalrempuia; Frøystein, Nils Åge et al. (2018). Steric factors on unsymmetrical O-hydroxyaryl N-Heterocyclic carbene ligands prevailing the stabilization of single stereoisomer of bis-ligated titanium complexes. (external link)
- Hahn, Friedrich; Setz, Christian; Friedrich, Melanie et al. (2014). Mutation of the highly conserved ser-40 of the HIV-1 p6 gag protein to phe causes the formation of a hydrophobic patch, enhances membrane association, and polyubiquitination of gag. (external link)
- Skår, Hjørdis; Seland, John Georg; Liang, Yucang et al. (2013). Screening of the relaxivity of gadolinium-loaded periodic mesoporous silica functionalized by means of soft metalorganic silylamide grafting. (external link)
- Evjenth, Rune Henrik; Brenner, Annette; Thompson, Paul R. et al. (2012). Human Protein N-terminal Acetyltransferase hNaa50p (hNAT5/hSAN) Follows Ordered Sequential Catalytic Mechanism (combined kinetic and NMR study). (external link)
- Brenner, Annette; Kieffer, Bruno; Trave, Gilles Michel et al. (2012). Thermal stability of chicken brain alpha-spectrin repeat 17: a spectroscopic study. (external link)
- Occhipinti, Giovanni; Jensen, Vidar Remi; Törnroos, Karl Wilhelm et al. (2009). Synthesis of a new bidentate NHC–Ag(I) complex and its unanticipated reaction with the Hoveyda–Grubbs first generation catalyst. (external link)
- Alvheim, Cecilie; Frøystein, Nils Åge; Vinje, Jo et al. (2009). Cytotoxic trans-oriented iminoether platinum complexes - Kinetics of binding to DNA oligonucleotides determined by 15-N NMR spectroscopy. (external link)
- Sommerfeldt, Hanne-Marthe; Meerman, Christian; Schrems, Marcus et al. (2008). Characterization and reactivity of peralkylated Ln(II)Al(III) heterobimetallic complexes. (external link)
- Zimmermann, Melanie; Frøystein, Nils Åge; Fischbach, Andreas et al. (2007). Homoleptic rare-earth metal(III) tetramethylaluminates: Structural chemistry, reactivity, and performance in isoprene polymerization. (external link)
- Grove, Hilde; Frøystein, Nils Åge; Sæthre, Leif J et al. (2006). Crystalline products isolated from solutions with commercially available 2,3-bis(2-pyridyl)pyrazine (dpp) as reactant: Detection of a dimerized form of dpp. (external link)
- Halskau, Øyvind; Underhaug, Jarl; Frøystein, Nils Åge et al. (2005). Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. (external link)
- Teigen, Knut; Dao, Khanh Kim; Mckinney, Jeffrey Alan et al. (2004). Tetrahydrobiopterin binding to aromatic amino acid hydroxylases. Ligand recognition and specificity. (external link)
- Doudin, K.; Berge, Rolf Kristian; Frøystein, Nils Åge et al. (2000). Preparation and NMR studies of 3-selenaheptadecanoic and 4-selenaoctadecanoic acid, RSe(CH2)nC(O)OH (R = -C14H29, n = 1 and 2), and their methyl esters. (external link)
- Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora (1999). The structural Basis of the Recognition of Phenylalanine and Pterine Cofactors by Phenylalanine Hydroxylase. Implications for the Catalytic Mechanism. (external link)
- Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora (1999). The structural basis of the recognition of phenylalanine and pterin cofactors by phenylalanine hydroxylase. Implications for the catalytic mechanism. (external link)
- Moldrheim, Erlend; Andersen, Bjørn; Frøystein, Nils Åge et al. (1998). Interaction of Manganese (II)Cobalt(II) and Nickel(I) with DNA Oligomers Studied by Proton NMR Spectroscopy. (external link)
- Fossen, Torgils; Frøystein, Nils Åge; Andersen, Øyvind Moksheim (1998). Myricetin 3-rhamnopyranosyl (1-6) galactoside from Nymphaea x marliacea. (external link)
- Garoufis, A.; Perlepes, S.P.; Frøystein, Nils Åge et al. (1996). Mercuration of acetone in the acetone-mercury(II) perchlorate2-('2-pyridyl)quinoxaline(L) system. (external link)
- Frøystein, Nils Åge; Sletten, Einar (1994). The binding of mercury(II) to the syntetic oligonucleotide d(CGcGAATTCGCG)%cA %1H adn %1%5N NMR study. (external link)
- Frøystein, Nils Åge; Davis, J. T.; Reid, B. R. et al. (1993). Sequence-Selective Metal Ion Binding to DNA Oligonucleotides. (external link)
- Steinkopf, Signe; Frøystein, Nils Åge; Liu, Q. et al. (1992). Copper(II) - Purine Complexes Studied by Proton and Nitrogen-15 Nuclear Magnetic Resonance. (external link)
- Stavropoulos, George; Karagiannis, Kostas; Vynios, Dimitrios et al. (1991). Solid-Phase Synthesis and Spectroscopic Studies of TRH Analogues Incorporating Cis- and trans-4-Hydroxy-L-Proline. (external link)
Short communication
Academic chapter/article/Conference paper
- Sletten, Einar; Frøystein, Nils Åge (2009). Sequence-Selective Binding of Transition Metal Complexes to DNA. (external link)
- Sletten, Einar; Frøystein, Nils Åge (1996). NMR Studies of Oligonucleotide-Metal Ion Interactions. (external link)
- Sletten, Einar; Frøystein, Nils Åge (1996). NMR studies of oligonucleotide - metal ion complexes. (external link)
- Sletten, Einar; Frøystein, Nils Åge (1995). NMR spectroscopic structure determination of metal ion - oligonucleotide complexes - the results of sequence-selective binding studies. (external link)
Academic lecture
- Sletten, Einar; Frøystein, Nils Åge (2008). NMR kinetic studies using isotope labeled reagents. (external link)
- Teigen, Knut; Frøystein, Nils Åge; Martínez, Aurora (1998). NMR study on the conformation of L-phenylalanine and dihydrobiopterin bound to wild-type and mutant forms of recombinant human phenylalanine hydroxylase. (external link)