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The sequencing facility

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DNA sequencing

 

Automated Sanger DNA Sequencing is performed using capillary-based Applied Biosystem 3730XL Analyzer. The instrument offers high throughput and sensitivity. Samples of single stranded, double stranded DNA, and PCR products, can be sequenced with reads >800 base pairs.

 

The sequencing PCR is performed by the user with high-quality template DNA, 1 primer, BigDye terminator 3.1 mix and sequencing buffer.

The clean-up of DNA at the sequencing lab is robotized.

Sequences are usually available overnight on our server.