Utvalgte publikasjoner

Heathcote KC, Keeley TP, Myllykoski M, Lundekvam M, McTiernan N, Akter S, Masson N, Ratcliffe PJ, Arnesen T, Flashman E (2024) N-terminal cysteine acetylation and oxidation patterns may define protein stability. Nature Commun 15(1):5360. 

Chelban V, Aksnes H, Maroofian R, LaMonica LC, Seabra L, Siggervåg A, Devic P, Shamseldin HE, Vandrovcova J, Murphy D, Richard AC, Quenez O, Bonnevalle A, Zanetti MN, Kaiyrzhanov R, Salpietro V, Efthymiou S, Schottlaender LV, Morsy H, Scardamaglia A, Tariq A, Pagnamenta AT, Pennavaria A, Krogstad LS, Bekkelund ÅK, Caiella A, Glomnes N, Brønstad KM, Tury S, Moreno De Luca A, Boland-Auge A, Olaso R, Deleuze JF, Anheim M, Cretin B, Vona B, Alajlan F, Abdulwahab F, Battini JL, İpek R, Bauer P, Zifarelli G, Gungor S, Kurul SH, Lochmuller H, Da'as SI, Fakhro KA, Gómez-Pascual A, Botía JA, Wood NW, Horvath R, Ernst AM, Rothman JE, McEntagart M, Crow YJ, Alkuraya FS, Nicolas G; SYNaPS Study Group; Arnesen T, Houlden H (2024) Biallelic NAA60 variants with impaired n-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications Nature Commun 15(1):2269. 

Kind L, Molnes J, Tjora E, Raasakka A, Myllykoski M, Colclough K, Saint-Martin C, Adelfalk C, Dusatkova P, Pruhova S, Valtonen-André C, Bellanné-Chantelot C, Arnesen T, Kursula P, Njølstad PR (2024) Molecular mechanism of HNF-1A mediated HNF4A gene regulation and promoter-driven HNF4A-MODY diabetes. JCI Insight 9(11):e175278.

Arnesen T, Kjosås I, McTiernan N (2024) Protein N-terminal acetylation is entering the degradation end game Nat Rev Mol Cell Biol 25(5):335-336. 

Varland S, Silva RD, Kjosås I, Faustino A, Bogaert A, Billmann M, Boukhatmi H, Kellen B, Costanzo M, Drazic A, Osberg C, Chan K, Zhang X, Tong AHY, Andreazza S, Lee JJ, Nedyalkova L, Ušaj M, Whitworth AJ, Andrews BJ, Moffat J, Myers CL, Gevaert K, Boone C, Martinho RG, Arnesen T (2023) N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity Nature Commun 14(1):6774.

Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T, Olsen JV (2023) Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae Nature Commun 14(1):4517

Aksnes H, McTiernan N, Arnesen T (2023) NATs at a glance J Cell Sci 136 (14): jcs260766

Van Damme P, Osberg C, Jonckheere V, Glomnes N, Gevaert K, Arnesen T, Aksnes H (2023) Expanded in vivo substrate profile of the yeast N-terminal acetyltransferase NatC.  J Biol Chem 299(2):102824

Arnesen T, Aksnes H, Giglione C (2023) Protein Termini 2022: central roles of protein ends Trends Biochem Sci 48(6):495-499

Kind L, Raasakka A, Molnes J, Aukrust I, Bjørkhaug L, Njølstad PR, Kursula P, Arnesen T (2022) Structural and biophysical characterization of transcription factor HNF-1A as a tool to study MODY3 diabetes variants. J Biol Chem 298(4):101803

McTiernan N, Tranebjærg L, Bjørheim AS, Hogue JS, Wilson WG, Schmidt B, Boerrigter MM, Nybo ML, Smeland MF, Tümer Z, Arnesen T (2022) Biochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N‑terminal acetylation. Hum Genet 141(8):1355-1369. 

Drazic A, Timmerman E, Kajan U, Marie M, Varland S, Impens F, Gevaert K, Arnesen T (2022) The final maturation state of β-actin involves N-terminal acetylation by NAA80 not N-terminal arginylation by ATE1. J Mol Biol 434(2):167397.

Morrison J, Altuwaijri NK, Brønstad K, Aksnes H, Alsaif HS, Evans A, Hashem M, Wheeler PG, Webb BD, Alkuraya FS, Arnesen T (2021) Missense NAA20 variants impairing the NatB protein N-terminal acetyltransferase cause autosomal recessive developmental delay, intellectual disability, and microcephaly. Genet Med 23(11):2213-2218. 

Kweon HY, Lee MN, Dorfel M, Seo S, Gottlieb L, PaPazyan T, McTiernan N, Ree R, Bolton D, Garcia A, Flory M, Crain J, Sebold A, Lyons S, Ismail A, Marchi E, Sonn SK, Jeong SJ, Jeon S, Ju S, Conway SJ, Kim T, Kim HS, Lee C, Roh TY, Arnesen T, Marmorstein R, Oh GT, Lyon GJ (2021) Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway. eLife 10:e65952.

Ward TL, Tai W, Morton SU, Impens F, VanDamme P, Van Haver D, Timmerman E, Venturini G, Zhang K, Jang MY, Willcox JA, Haghighi A, Gelb BD, Chung WK, Goldmuntz E, Porter GA, Lifton R, Brueckner M, Yost HJ, Bruneau BG, Gorham J, Kim Y, Pereira AC, Homsy J, Benson CC, DePalma SR, Varland S, Chen CS, Arnesen T, Gevaert K, Seidman CE, Seidman JG (2021) Mechanisms of Congenital Heart Disease Caused by NAA15 Haploinsufficiency. Circ Res 128(8):1156-1169. Commentary by Timothy Cashman and Chinmay Trivedi (2021) N-Acetyl Transferases - New Insights Into Human Congenital Cardiovascular Defects. Circ Res 128(8):1170–1172. 

McTiernan N, Gill H, Prada CE, Pachajoa H, Lores J, CAUSES study, Arnesen T (2021) NAA10 p.(N101K) disrupts N-terminal acetyltransferase complex NatA and is associated with developmental delay and hemihypertrophy Eur J Hum Genet 29(2):280-288.

Deng S, McTiernan N, Wei X, Arnesen T, Marmorstein R (2020) Molecular basis for N-terminal acetylation by human NatE and its modulation by HYPK Nature Commun 11:818.

Rebowski G, Boczkowska M, Drazic A, Ree R, Goris M, Arnesen T, Dominguez R (2020) Mechanism of actin N-terminal acetylation Science Adv 6:eaay8793.

Ree R, Kind L, Kaziales A, Varland S, Dai M, Richter K, Drazic A, Arnesen T (2020) PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin. J Biol Chem 295(49):16713-16731.

Beigl T, Hellesvik M, Saraste J, Arnesen T, Aksnes H (2020) N-terminal acetylation of actin by NAA80 is essential for structural integrity of the Golgi apparatus Exp Cell Res 390(2):111961.

Krtenic B, Drazic A, Arnesen T, Reuter N (2020) Classification and phylogeny for the annotation of novel eukaryotic GNAT acetyltransferases. PLoS Comp Biol 16(12):e1007988. 

Aksnes H, Ree R, Arnesen T (2019) Co-translational, post-translational and non-catalytic roles of N-terminal acetyltransferases Mol Cell 73(6):1097-1114.

Drazic A, Aksnes H, Marie M, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T (2018) NAA80 is actin’s N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility Proc Natl Acad Sci USA 115(17):4399-4404.   Commented in: NATure of actin amino-terminal acetylation Proc Natl Acad Sci USA 115 (17) 4314-4316; Actin's N-terminal acetyltransferase uncovered Cytoskeleton (Hoboken) doi: 10.1002/cm.21455. 

Goris M, Magin RS, Foyn H, Myklebust LM, Varland S, Ree R, Drazic A, Bhambra P, Støve SI, Baumann M, Haug BE, Marmorstein R, Arnesen T (2018) Structural determinants and cellular environment define processed actin as the sole substrate of the N-terminal acetyltransferase NAA80 Proc Natl Acad Sci USA 115(17):4405-4410.  

Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T (2018) N-terminal acetylation levels are maintained during acetyl-CoA deficiency in Saccharomyces cerevisiae Mol Cell Proteomics 17(12):2309-2323.

Ree R, Varland S, Arnesen T (2018) Spotlight on protein N-terminal acetylation Exp Mol Med 50(7):90.

Cheng H, Dharmadhikari AV, Varland S, Ma N, Domingo D, Kleyner R, Rope AF, Yoon M, Stray-Pedersen A, Posey JE, Crews SR, Eldomery MK, Akdemir ZC, Lewis AM, Sutton VR, Rosenfeld JA, Conboy E, Agre K, Xia F, Walkiewicz M, Longoni M, High FA, van Slegtenhorst MA, Mancini GMS, Finnila CR, van Haeringen A, den Hollander N, Ruivenkamp C, Naidu S, Mahida S, Palmer EE, Murray L,  Lim D, Jayakar P, Parker MJ, Giusto S, Stracuzzi E, Romano C, Beighley JS, Bernier RA, Küry S, Nizon M, Corbett MA, Shaw M, Gardner A, Barnett C, Armstrong R, Kassahn KS, Van Dijck A, Vandeweyer G, Kleefstra T, Schieving J, Jongmans MJ, de Vries BBA, Pfundt R, Kerr B, Rojas SK, Boycott KM, Person R, Willaert R, Eichler EE, Kooy F, Yang Y, Wu JC, Lupski JR, Arnesen T, Cooper GM, Chung WK, Gecz J, Stessman HAF, Meng L, Lyon GJ (2018) Truncating Variants in NAA15 Are Associated with Variable Levels of Intellectual Disability, Autism Spectrum Disorder, and Congenital Anomalies Am J Hum Genet DOI: 10.1016/j.ajhg.2018.03.004 

Aksnes H, Goris M, Strømland Ø, Drazic A, Waheed Q, Reuter N, Arnesen T (2017) Molecular determinants of the N-terminal acetyltransferase Naa60 anchoring to the Golgi membrane J Biol Chem 292(16):6821-6837.

Aksnes H, Drazic A, Marie M, Arnesen T (2016) First Things First: Vital Protein Marks by N-Terminal Acetyltransferases. Trends Biochem Sci 41(9):746-60.

Støve SI, Magin R, Foyn H, Haug BE, Marmorstein R, Arnesen T (2016) Crystal structure of the Golgi-associated human N-alpha acetyltransferase 60 reveals the molecular determinants for substrate-specific acetylation. Structure 24(7):1044-56.

Van Damme P, Kalvik TV, Starheim KK, Jonckheere V, Myklebust LM, Menschaert G, Varhaug JE, Gevaert K, Arnesen T (2016) A role for human N-terminal acetyltransferase Naa30 in maintaining mitochondrial integrity. Mol Cell Proteomics 15(11):3361-3372.

Saunier C, Støve SI, Popp B, Gérard B, Blenski M, AhMew N, de Bie C, Goldenberg P, Isidor B, Keren B, Leheup B, Lampert L, Mignot C, Tezcan K, Mancini GM, Nava C, Wasserstein M, Bruel AL, Thevenon J, Masurel A, Duffourd Y, Kuentz P, Huet F, Rivière JB, van Slegtenhorst M, Faivre L, Piton A, Reis A, Arnesen T, Thauvin-Robinet C, Zweier C (2016) Expanding the Phenotype Associated with NAA10 Related N-terminal Acetylation Deficiency. Hum Mutat 37(8):755-64.

Drazic A, Myklebust LM, Ree R, Arnesen T (2016) The world of protein acetylation. Biochim Biophys Acta 1864(10):1372-401.

Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, Støve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T (2015) An Organellar N-alpha Acetyltransferase, Naa60, Acetylates Cytosolic N-termini of Transmembrane Proteins and Maintains Golgi Integrity. Cell Rep 10(8):1362-74. 

Linster E, Stephan I, Bienvenut WV, Maple-Grødem J, Myklebust LM, Huber M, Reichelt M, Sticht C, Geir Møller S, Meinnel T, Arnesen T, Giglione C, Hell R, Wirtz M (2015) Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nature Commun 6:7640.

Myklebust LM, Van Damme P, Støve SI, Dörfel MJ, Abboud A, Kalvik TV, Grauffel C, Jonckheere V, Wu Y, Swensen J, Kaasa H, Liszczak G, Marmorstein R, Reuter N, Lyon GJ, Gevaert K, Arnesen T (2015) Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-acetylation defects. Hum Mol Genet 24(7):1956-76.

Ree RM, Myklebust LM, Thiel P, Foyn H, Fladmark KE, Arnesen T (2015) The N-terminal acetyltransferase Naa10 is essential for zebrafish development. Biosci Rep 35(5). pii: e00249.

Van Damme P, Hole K, Gevaert K, Arnesen T (2015) N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases. Proteomics 15(14):2436-46.

Varland S, Osberg C, Arnesen T (2015) N-terminal modifications of cellular proteins: the enzymes involved, their substrate specificities and biological effects. Proteomics 15(14):2385-401.

Van Damme P, Støve SI, Glomnes N, Gevaert K, Arnesen T (2014) A Saccharomyces cerevisiae model reveals in vivo functional impairment of the Ogden syndrome N-terminal acetyltransferase Naa10S37P mutant. Mol Cell Proteomics 13(8):2031-41.

Liszczak G, Goldberg JM, Foyn H, Petersson EJ, Arnesen T, Marmorstein R (2013) Molecular basis for N-terminal acetylation by the heterodimeric NatA complex. Nature Struct Mol Biol 20(9):1098-105.

Foyn H, Jones JE, Lewallen D, Narawane R, Varhaug JE, Thompson PR, Arnesen T (2013) Design, Synthesis, and Kinetic Characterization of Protein N-Terminal Acetyltransferase Inhibitors. ACS Chem Biol 8:1121-1127. 

Kalvik TV and Arnesen T (2013) Protein N-terminal acetyltransferases in cancer. Oncogene 32:269–276.

Foyn H, Van Damme P, Støve S, Glomnes N, Evjenth R, Gevaert K, Arnesen T (2013) Protein N-Terminal acetyltransferases act as N-Terminal propionyltransferases in vitro and in vivo. Mol Cell Proteomics 12:42-54. Covered by ASBMB news

Starheim KK, Gevaert K, Arnesen T (2012) Protein N-terminal acetyltransferases: when the start matters. Trends Biochem Sci 37(4):152-61. Selected by the Editorial Board to the top ten TiBS articles of 2012.

Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci USA 109(31):12449-54.

Arnesen T (2011) Towards a functional understanding of protein N-terminal acetylation. PLoS Biology 9(5):e1001074. 

Rope A, Wang K, Evjenth R, Xing J, Johnston JJ, Swensen JJ, Johnson WE, Moore B, Huff CD, Bird LM, Carey JC, Opitz JM, Stevens CA, Jiang T, Schank C, Fain HD, Robison R, Dalley B, Chin S, South ST, Pysher TJ, Jorde LB, Hakonarson H, Lillehaug JR, Biesecker LB, Yandell M, Arnesen T, Lyon GJ (2011) Using VAAST to Identify an X-Linked Disorder Resulting in Lethality in Male Infants Due to N-Terminal Acetyltransferase Deficiency. Am J Hum Genet 89(1):28-43. Comment in Nature News: doi:10.1038/news.2011.382 

Van Damme P, Hole K, Pimenta-Marques A, Helsens K, Vandekerckhove J, Martinho RG, Gevaert K, Arnesen T (2011) NatF Contributes to an Evolutionary Shift in Protein N-Terminal Acetylation and Is Important for Normal Chromosome Segregation. PLoS Genet 7(7):e1002169.

Liszczak G, Arnesen T, Marmorstein R (2011) Structure of a ternary Naa50p (NAT5/SAN) Nα-terminal acetyltransferase complex reveals the molecular basis for substrate specific acetylation. J Biol Chem 286(42):37002-10. 

Van Damme P, Evjenth R, Foyn H, Demeyer K, De Bock P-J, Lillehaug JR, Vandekerckhove J, Arnesen T, Gevaert K (2011) Proteome-derived peptide libraries allow detailed analysis of the substrate specificities of Nα-acetyltransferases and point to hNaa10p as the posttranslational actin Nα-acetyltransferase. Mol Cell Proteomics  10:M110.004580.  

Arnesen T, Starheim KK, Van Damme P, Evjenth R, Dinh H, Betts M, Ryningen A, Vandekerckhove J, Gevaert K, Anderson D (2010) The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation. Mol Cell Biol 30(8):1898-909.

Arnesen T, Van Damme P, Polevoda B, Helsens K, Evjenth R, Colaert N, Varhaug JE, Vandekerckhove J, Lillehaug JR, Sherman F, Gevaert K (2009) Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans. Proc Natl Acad Sci USA 106:8157-8162.

Starheim K, Gromyko D, Ryningen A, Varhaug JE, Lillehaug JR, Arnesen T (2009) Knockdown of the human NatC protein Nα-terminal acetyltransferase complex induces p53-mediated apoptosis and aberrant hArl8b localization. Mol Cell Biol 29(13):3569-81.

Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR (2009) Human Naa50 (Nat5/San) displays both protein N-alpha and N-epsilon acetyltransferase activity. J Biol Chem. 284(45):31122-9. 

Arnesen T, Gromyko D, Pendino F, Ryningen A, Varhaug JE, Lillehaug JR. (2006) Induction of apoptosis in human cells by RNAi mediated knock-down of hARD1 and NATH, components of the protein N-α-acetyltransferase complex. Oncogene 25:4350-4360. 

Arnesen T, Anderson D, Baldersheim C, Lanotte M, Varhaug JE, Lillehaug JR. (2005) Identification and characterization of the human ARD1-NATH protein acetyltransferase complex. Biochemical J 386(Pt 3):433-43.