Varselmelding

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What is a BigDye blob?

Unincorporated nucleotides can form a "BigDye blob", a double blue-red C/T peak around nucleotide 80. It is a common feature with this chemistry.The blob can be at background level, or override the sequence. One might be able to read the sequence through the blob as shown in the picture:

Hovedinnhold

21.03.2013