Course in basic microscopy 2023
MIC is happy to announce a compact three day course in basic microscopy, with main focus on techniques based on fluorescence. This course will kick start student/scientists new to the field of imaging and help them navigate techniques and optimize their imaging experiments.
Main content
The Molecular Imaging Center (MIC) will arrange a Course in basic microscopy 19.-21. September 2023. This is a compact course aimed at new beginners in the field of microscopy, and at those with some experience in search of a better understanding of the principles. The course introduces mainly techniques that involve samples with fluorescent markers, but also regular bright field imaging will be covered.
Microscopy is very technology driven and there is a huge range of technologies/systems that can be used for many different applications that involve both simple and advanced imaging experiments. In this course the focus will be on the most basic (= most common) applications on a selection of different microscopy systems. These systems are all accessible to students/scientists through MIC, and hopefully the course will equip the students with more knowledge on which system to choose over another in future imaging experiments.
During the course there will be demos on five different systems. Students will still need additional training on the microscopes they want to start using, but after such a course their basic understanding of these powerful tools will be at a different level, and hopefully help them perform imaging experiments with higher quality from the beginning.
Descriptive images can be useful, but in most cases image processing and analysis will become necessary. It is important to understand which information microscopy images contains and how to handle them. It is a great benefit to start using image analysis software early in an imaging career, so a brief introduction will be given of the two most commonly used, Fiji (ImageJ) and QuPath. Both are developed by and for scientists, and free to download.
Register here. Limited number of participants.
Course fee: 3000 NOK (inkl. course material).
Program (changes may occur):
Day 1 | Day 2 | Day 3 |
0900-0930 Welcome, intro and quiz 0930-1030 Basics of microscopy 1030-1045 Break 1045-1145 Basic live cell imaging, sample preparation, fluorescent proteins 1145-1230 Lunch 1230-1315 Examples and planning of imaging experiments. 1315-1330 Break 1330-1500 Demo 1 (fluorescence microscope / slide scanner) 1500-1515 Break 1515-1645 Demo 2 (fluorescence microscope / slide scanner) | 0900-1000 Basics of confocal 1000-1015 Break 1015-1115 A digital image 1115-1215 Lunch 1215 -1415 Demo 3 (scanning confocal/spinning disk confocal) 1415-1430 Break 1430-1630 Demo 4 (scanning confocal/spinning disk confocal)
| 0900-1030 Demo IncuCyte with data analysis 1030-1045 Break 1045-1115 Data management 1115-1215 Lunch 1215-1300 Intro to Fiji/ImageJ 1300-1400 Fiji/ImageJ continues (E) 1400-1415 Break 1415-1530 Intro to QuPath (H) 1530-1600 Summary and TEST
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