Current projects overview

1. Identifying phosphoinositide interacting nuclear proteins to understand their function

In order to decipher phosphoinositide (PPIn) nuclear functions, we have established quantitative proteomic methods to enrich for and to identify nuclear PPIn-effector proteins. Using this method, we have identified nuclear proteins interacting with phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2) and PtdIns(3,4,5)P3. Current projects include further analyses of these potential interactions:

• Biochemical validation of the interactions
• Role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 interaction in the function of these proteins in mRNA processing and nucleolar processes)
• Mechanisms of regulation of these interactions

2. Mapping altered phosphoinositide-protein interactomes in cancer

Our goal is to map which protein-PPIn networks are remodeled in pathological states where the PPIn metabolic pathways are known to be altered, in particular in endometrial cancer