Tumours
Isolation of interstitial fluid in tumours has recently been successful in tumours (and skin serving as a reference) using centrifugation and an increased G-force. Recently we have become interested in the role of the lymphatics in fluid volume regulation.
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Studies in experimental carcinomas have demonstrated that the elevated Pif can be lowered by the same agents that cause a lowering of Pif in acute inflammatory reactions and can be used to enhance the delivery of cytostatic agents and thus slow the tumour growth. Studies of tumour oxygenation have focused on the role in tumour pathophysiology, for acquired treatment resistance, and tumour progression by comparing the effects of hypoxia and hyperoxia (the “flip of a coin”) both in vivo and in vitro using model systems and with a strong focus on the role of the interstitial factors. We have shown that elevating the pO2 in the tumour tissue using hyperbaric oxygen has a significant impact on chemically induced mammary tumours and transplanted gliomas. Hyperoxia will lower tumour growth, angiogenesis, proliferation, apoptosis and raise drug-uptake and metabolism, and induce endothelial-to-mesenchymal transion by altering whole gene programs. A model using dsRed labelled tumour cells injected to GFP mice has been developed allowing separation of tumour cells from the host-derived stromal cells.