BBB Seminar: Clive D'Santos

Clive D'Santos
PROBE Proteomic Platform, Department of Biomedicine, University of Bergen

Mass spectrometry-based proteomics techniques have evolved dramatically over the last 10 years or more and have reached a level of maturity reflected in numerous high impact publications which utilize this burgeoning field of science. Better sample preparation procedures, radical developments in instrumentation and improved software have all contributed to this success. Areas in which proteomics have made considerable progress include the ability to characterise and understand post-translational modifications (PTMs) and protein-protein interactions in cellular functions such as signalling pathways. In this talk data will be presented which addresses both of these areas.

(1) The identification of a novel PTM: propionylation. Propionylation has been found to occur on key signalling proteins such as histone, the tumour suppressor protein p53 and the acetyl transferase p300. Using MS and a bioinformatics approach we identify here sites of modification and speculate on the role of this novel PTM.

(2) A comparative SILAC-based methodology will be described which aims to clarify the role(s) of phosphatidylinositol phosphate lipid signalling molecules within the context of the mammalian nucleus.

Chair: Jaakko Saraste (jaakko.saraste@uib.no), Department of Biomedicine