Instruments
The laboratory is located at the Laboratory Building, 8th floor, Haukeland University Hospital
Main content
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) is a bioanalytical technique combining the separation capabilities of liquid chromatography with the sensitivity and specificity of tandem mass spectrometry. In LC-MS/MS, liquid chromatography separates complex mixtures of compounds, while tandem mass spectrometry provides precise identification and quantification by analyzing the mass-to-charge ratio of ions. LC-MS/MS offers high accuracy and sensitivity for complex samples and is widely used for detailed chemical analysis such as drug discovery, biomarker detection and environmental monitoring.
The Core Facility operates three LC-MS/MS units from Waters that are used for sample analysis (Figure 1). The instruments can determine masses up to approximately 2000 Da and have a mass resolution of just under 1 Da. They are state-of-the-art in terms of analytical sensitivity, but the sensitivity is substance-dependent.
Sample preparation is automated and performed on a Hamilton STAR pipetting robot (Figure 2). The robot has 8 channels and can precisely pipette in the range of 0.5–1,000 µL.
The laboratory is fully equipped with various smaller equipment neccessary for routine LC-MS/MS analysis and method development.