Prof. Clive Bramham and Adrian Szum, Neuroscience Research Group
Studies of the cellular and molecular mechanisms of long-term synaptic plasticity in the normal brain and dysregulation in brain disorders.
Live 4D confocal imaging of rat hippocampal neurons transfected with the photoswitchable fluorescent protein Dendra2, showing complex neuronal networks between cells. The live neurons were acquisitioned on the Leica TCS SP5 confocal microscope system and rendered in Bitplane Imaris.
Photo:
Adrian Szum
Main content
A major goal is to elucidate the regulation and function of Arc as a master gene in synaptic plasticity and memory formation, and how mutations in human Arc affect these mechanisms. Cellular imaging techniques play a central role in our multidisciplinary efforts. At MIC, we use the Leica SP5 confocal microscope for time-lapse imaging in primary hippocampal neuronal cultures.
Link to movies:
GluR surface staining of hippocampal neurons.
Confocal imaging of rat Hippocampal neurons transfected with Dendra2.
03.01.2014